协和医学杂志

2012, (01)

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全自动DNA定量分析系统在胸腔积液细胞病理诊断中应用的研究
To study the application of Automatic DNA image cytometry in diagnosis of pleural effusion

孟芝兰

摘要(Abstract):

目的 探讨细胞DNA定量分析技术在胸腔积液细胞病理诊断中的应用。方法 采用细胞DNA定量分析技术对50例胸腔积液脱落细胞DNA 含量进行检测,并以细胞学诊断结果作为标准,分析该技术诊断阳性病例的最佳阈值。结果 该技术在胸腔积液诊断中,细胞DI值为2.5时判定为非整倍体细胞。该种类细胞数目最小值为1时诊断阳性的敏感性为87.5%,特异性为100%。结论 细胞DNA定量分析技术在胸腔积液诊断中有良好的应用前景,其诊断阈值需扩大样本量进一步验证。
[Abstract] Objective To study the application of DNA Image Cytometry in diagnosis of pleural effusion.Methods There are 50 available cases with a cytology diagnosis to be involved in this study. All the samples of these cases have been prepared by liquid-based method and stained with Feulgen staining. The slides have been scanned by MotiCytometer. Results Compared with exfoliative cytology examination, the sensitivity is 87.5% and specificity is 100% when the diagnosis threshold of DNA-ICM is 1 cell with DI≥2.5. Conclusion DNA- ICM can be used in diagnosis of pleural effusion, and the threshold of its diagnosis should be confirmed in the further study.

关键词(KeyWords): |细胞DNA定量分析| 胸腔积液| 细胞学诊断|
|DNA Image Cytometry( DNA-ICM)|pleural effusion|Cytological diagnosis|

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作者(Author): 孟芝兰

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