协和医学杂志

2012, (01) 36-40

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全自动DNA定量分析系统在胸腔积液细胞病理诊断中的应用
Application of Automatic DNA Image Cytometry in Diagnosis of Pleural Effusion

孟芝兰;高亮;顾建刚;罗玉凤;钟桃铃;朱晨雁;
MENG Zhi-lan1,GAO Liang2,GU Jian-gang1,LUO Yu-feng1,ZHONG Tao-ling2,ZHU Chen-yan2 1Department of Pathology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China 2 Motic Pathology Diagnostic Center,Xiamen 361006,China

摘要(Abstract):

目的探讨细胞DNA定量分析技术在胸腔积液细胞病理诊断中的应用价值。方法采用细胞DNA定量分析技术对50例胸腔积液脱落细胞DNA含量进行检测,并以细胞学诊断结果作为标准,分析该技术诊断阳性病例的最佳阈值。结果细胞DNA定量分析技术用于胸腔积液诊断,以细胞DNA指数(DNAindex,DI)2.5判定为非整倍体细胞,该类细胞数目最小值为1时诊断阳性敏感性为87.5%,特异性为100%。结论细胞DNA定量分析技术在胸腔积液诊断中有良好的应用前景,其诊断阈值需扩大样本量进一步验证。
Objective To study the application of DNA image cytometry(DNA-ICM) in the diagnosis of pleural effusion.Methods DNA-ICM was applied for the determination of DNA levels in the cast-off cells from pleural effusion in 50 patients.Meanwhile,using the cytological test results as the standard,we analyzed the optimal thresholds of this technology in diagnosing the positive cases.Results Compared with exfoliative cytology examination,DNA-ICM had a sensitivity of 87.5% and a specificity of 100% in the diagnosis,with a threshold of 1 cell when the DNA index ≥2.5.Conclusion DNA-ICM can be promising in the diagnosis of pleural effusion,whereas its thresholds require further investigations.

关键词(KeyWords): 细胞DNA定量分析;胸腔积液;细胞学诊断
DNA image cytometry;pleural effusion;cytological diagnosis

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作者(Author): 孟芝兰;高亮;顾建刚;罗玉凤;钟桃铃;朱晨雁;
MENG Zhi-lan1,GAO Liang2,GU Jian-gang1,LUO Yu-feng1,ZHONG Tao-ling2,ZHU Chen-yan2 1Department of Pathology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China 2 Motic Pathology Diagnostic Center,Xiamen 361006,China

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