协和医学杂志

2012, (01) 26-29

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Let-7a和miR-96对胰腺癌K-RAS基因的调节作用
Regulatory Effects of Let-7a and miR-96 Molecules on K-RAS in Pancreatic Cancer

于双妮;孟云霄;马怡晖;卢朝辉;陈杰;
YU Shuang-ni,MENG Yun-xiao,MA Yi-hui,LU Zhao-hui,CHEN Jie Department of Pathology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China

摘要(Abstract):

目的探讨miRNA分子Let-7a和miR-96对胰腺癌系K-RAS基因的抑制作用及其致病机制。方法通过检测胰腺癌细胞系miRNA分子(Let-7a和miR-96)水平、K-RAS基因mRNA和蛋白质表达水平,观察Let-7a和miR-96水平与K-RAS蛋白表达的关系;并通过升高胰腺癌细胞Let-7a和miR-96水平,检测其对K-RAS蛋白表达水平的影响。结果胰腺癌细胞系K-RAS的mRNA与蛋白质表达均保持在一个较高的水平;胰腺癌细胞系miRNA分子Let-7a和miR-96水平均较低;升高胰腺癌细胞Let-7a或miR-96水平会降低K-RAS蛋白表达水平。结论正常状态下,Let-7a和miR-96通过调控K-RAS基因发挥对肿瘤的抑制作用,胰腺癌细胞的Let-7a和miR-96水平呈一定程度的降低,削弱了对K-RAS基因的抑制作用,进而参与肿瘤的致病过程。
Objective To explore the effects of Let-7a and miR-96 molecules on inhibition K-RAS in pancreatic cancer.Methods The levels of K-RAS mRNA and protein were detected in 9 pancreatic cancer cell lines,and the abundance of Let-7a and miR-96 in 7 of these 9 cell lines were determined to analyze the relation between the levels of Let-7a or miR-96 and K-RAS protein expression.Then,the levels of Let-7a or miR-96 was increased in PANC-1 cell line by transfection to observe K-RAS protein change.Results A high level of K-RAS mRNA was observed in 9 pancreatic cancer cell lines,and K-RAS protein was also at a higher level than normal ones.But Let-7a and miR-96 maintained were at a lower level in pancreatic cancer cell lines.An increase of Let-7a or miR-96 decreased the K-RAS protein expression in PANC-1 cell line.Conclusion Compared to normal condition,the levels of Let-7a and miR-96 decrease in pancreatic cancer,leading to the attenuation of inhibition to K-RAS,which contributes to pancreatic cancer formation and progression.

关键词(KeyWords): 胰腺癌;Let-7a;miR-96;K-RAS
pancreatic cancer;Let-7a;miR-96;K-RAS

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作者(Author): 于双妮;孟云霄;马怡晖;卢朝辉;陈杰;
YU Shuang-ni,MENG Yun-xiao,MA Yi-hui,LU Zhao-hui,CHEN Jie Department of Pathology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China

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