协和医学杂志

2010, v.1(02) 145-149

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

两种溃疡性结肠炎小鼠模型的构建及其炎症反应机制比较
Establishment of Two Murine Models of Ulcerative Colitis and Comparison of the Mechanism of Colitis-related Inflammatory Reactions

苗政;钱家鸣;李景南;唐晓艳;杨晓鸥;
MIAO Zheng,QIAN Jia-ming,LI Jing-nan,TANG Xiao-yan,YANG Xiao-ou Department of Gastroenterology,Peking Union Medical College Hospital,Chinese Academyof Medical Science & Peking Union Medical College,Beijing 100730,China

摘要(Abstract):

目的构建葡聚糖硫酸钠(dextransulfatesodium,DSS)以及恶唑酮(oxazolone,OXZ)诱导的小鼠溃疡性结肠炎模型,比较两种模型中炎症因子水平的变化,评价两种模型。方法 12只C57BL小鼠,随机分为两组,DSS模型组(n=8)予3%DSS自由饮用5d,DSS对照组(n=4)饮用蒸馏水5d,第6天处死两组小鼠。8只BALB/C小鼠,随机分为两组,OXZ模型组(n=4)予皮肤涂抹3%OXZ(100%乙醇溶剂)0.2ml2d,5d后以0.8%OXZ(50%乙醇溶剂)0.15ml灌肠;OXZ对照组予皮肤涂抹100%乙醇0.2ml2d,5d后以50%乙醇0.15ml灌肠,灌肠后第3天处死两组小鼠。观察各组小鼠疾病活动指数(disease activity index,DAI)、结肠组织大体评分和病理评分,并检测小鼠结肠组织中髓过氧化物酶(myeloperoxidase,MPO)、白细胞介素-4(interleukin4,IL-4)、干扰素-γ(interferon-γ,IFN-γ)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、核因子κB(nuclear factor-Kappa B,NF-κB)的水平。结果两种模型组小鼠DAI、组织大体评分和病理评分均较对照组有明显改变;DSS结肠炎、OXZ结肠炎均可导致MPO、TNF-α、NF-κB明显上升,DSS结肠炎IFN-γ明显上升,OXZ结肠炎IL-4明显上升。结论 DSS及OXZ均能诱导出小鼠溃疡性结肠炎模型,其中OXZ诱导的小鼠溃疡性结肠炎是Th2型炎症反应,更接近人类溃疡性结肠炎。
Objective To establish two murine models of ulcerative colitis (UC) and explore the mechanism of colitits-related inflammatory reactions. Methods Totally 12 C57BL mice were randomly divided into two groups:dextransulfatesodium (DSS) model group (n=8,allowed to drink 3% DSS for 5 days) and DSS control group (n=4,allowed to drink water for 5 days). In addition,8 BALB/C mice were randomly divided into oxazolone (OXZ) model group(n=4,mice were skin-sensitized with 3% OXZ 0.2 ml in 100% ethanol for 2 days followed by intrarectal administration of 0.8% OXZ 0.15 ml in 50% ethanol 5 days later) and OXZ control group(n=4,mice were skin-sensitized with 100% ethanol for 2 days followed by intrarectal administration of 50% ethanol 0.15 ml 5 days later). The disease activity index (DAI),tissular general score,and histological score were calculated. The levels of myeloperoxidase (MPO),interleukin 4 (IL-4),interferon-γ (INF-γ),tumor necrosis factor-α(TNF-α),and nuclear factor-Kappa B (NF-κB) in the colon tissue were determined. Results DAI,tissular general score,and histological score were significantly different in either DSS or OXZ model group when compared with their control groups. MPO,TNF-α,and NF-κB significantly increased in DSS and OXZ model groups. In addition,INF-γ significantly increased in DSS model group and IL-4 significantly increased in OXZ model group. Conclusions Both DSS and OXZ can induce ulcerative colitis in mice. OXZ-induced murine colitis is highly associated with T helper cell type 2 (Th2),which is more similar to human UC.

关键词(KeyWords): 溃疡性结肠炎;葡聚糖硫酸钠;恶唑酮;白细胞介素-4;干扰素-γ;肿瘤坏死因子-α;核因子κB
ulcerative colitis; dextransulfatesodium; oxazolone; interleukin 4; interferon-γ; tumor necrosis factor-α; nuclear factor-Kappa B

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 苗政;钱家鸣;李景南;唐晓艳;杨晓鸥;
MIAO Zheng,QIAN Jia-ming,LI Jing-nan,TANG Xiao-yan,YANG Xiao-ou Department of Gastroenterology,Peking Union Medical College Hospital,Chinese Academyof Medical Science & Peking Union Medical College,Beijing 100730,China

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享