协和医学杂志

2011, v.2(01) 1-6

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钙结合蛋白及相关蛋白在脊柱侧凸患者椎间盘终板软骨中的表达
Expressions of Calcium-binding Protein and Related Protein in the Endplate Cartilage of Patients with Idiopathic Scoliosis

吴志宏;程海燕;吴居泰;王以朋;邱贵兴;
WU Zhi-hong,CHENG Hai-yan,WU Ju-tai,WANG Yi-peng,QIU Gui-xing Department of Orthopaedics,Peking Union Medical College Hospital,Chinese Academy of Medical Science & Peking Union Medical College,Beijing 100730,China

摘要(Abstract):

目的观察钙结合蛋白S100A8、热休克蛋白(heat shock protein,HSP)90和钙调蛋白(calmodulin,CaM)基因在特发性脊柱侧凸(idiopathic scoliosis,IS)患者和先天性脊柱侧凸(congenital scoliosis,CS)患者椎间盘终板内的表达。方法收集经临床和影像学诊断为特发性脊柱侧凸的前路脊柱手术患者的椎间盘终板和先天性脊柱侧凸患者的椎间盘终板各4例,用TRIzol法提取终板软骨中的总蛋白,采用Western blot技术分析和对比椎间盘终板中S100A8、HSP90和CaM基因的表达。结果 S100A8在特发性脊柱侧凸和先天性脊柱侧凸患者中的表达有显著性差异,而CaM和HSP90则没有明显差别。结论 S100A8基因在特发性脊柱侧凸发生发展过程中起到一定的作用,需对该基因的表达和调控做进一步研究。
Objective To observe the expression of S100A8,heat shock protein(HSP)90,and calmodulin(CaM)in the discs endplate cartilage of patients with idiopathic scoliosis(IS)and congenital scoliosis(CS).Methods The discs of 4 IS patients(diagnosed by clinical and radiological data)and 4 CS patients who had undergone anterior-route surgeries were collected.Total protein was extracted from endplate cartilage with TRIzol method.The expressions of S100A8,HSP90,and CaM genes in the cartilages were analyzed using Western blot.Results The expressions of HSP90 and CaM in the cartilages were not significantly different between CS patients and IS patients,while the expression of S100A8 was significantly higher in IS patients than that in CS patients.Conclusion S100A8 may play a role in the occurrence and development of IS.

关键词(KeyWords): 脊柱侧凸;终板;S100A8;热休克蛋白90;钙调蛋白
scoliosis;endplate;S100A8;heat shock protein 90;calmodulin

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基金项目(Foundation): 国家自然科学基金(30271312,30471762)

作者(Author): 吴志宏;程海燕;吴居泰;王以朋;邱贵兴;
WU Zhi-hong,CHENG Hai-yan,WU Ju-tai,WANG Yi-peng,QIU Gui-xing Department of Orthopaedics,Peking Union Medical College Hospital,Chinese Academy of Medical Science & Peking Union Medical College,Beijing 100730,China

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